Excelsior Steam Biological Indicators

Excelsior offers spore ampoules for use in monitoring the efficacy of steam sterilization processes. Spore ampoules are ideal for use in validation of liquid sterilisation cycles. No activation is required; simply expose spore ampoule to sterilization process and incubate. Spore ampoules are available in several configurations.

All spore ampoules are hermetically sealed type I borosilicate glass containing a modified Soybean Casein Digest Broth with bromocresol purple pH indicator and the specified population of Geobacillus stearothermophilus spores. Growth is evident by either turbidity and/or a colour shift.

Biological indicator Spore Strips for monitoring steam processes consist of:

• An inoculated carrier, 6mm x 30mm thread of Bacillus subtilis (Cell Line 35021)
• Primary packaging in glassine envelopes

The Spore Strips are utilised to monitor Steam sterilisation process efficacy. Bacillus subtilis is typically used to monitor low temperature steam processes (100°C -120°C). The Spore Strips are labelled for industrial use only.

Process Steam Strip Dimensions: 6mm x 30 mm
Glassine Dimensions: 30mm x 38mm
Packaging: 100 / Pack

INOCULATED CARRIER SPORE DISCS For Monitoring STEAM

Disc dimensions: 6 mm

Packaging: 100 / Pack

Inoculated carrier Spore Discs and mini Spore Discs for monitoring steam processes consist of:

• An inoculated carrier, 6mm disc of Geobacillus stearothermophilus (Cell Line 7953)
• Primary packaging in bulk

The Spore discs are designed to directly into a device and used to monitor steam sterilisation process efficacy. The Spore Discs are labelled for industrial use only 

Spore Discs will fit into small areas of a device where a standard-sized (6 mm x 30 mm) spore strip cannot be used, such as within a syringe barrel, inside tubing or under a cap.

Excelsior Spore Discs are manufactured in shelf packs of 100 with an 18 or 24 month shelf-life, based on organism and date of manufacture, when stored under room temperature conditions. Discs are certified for species population, purity, resistance, (dvalue, zvalue, survival/kill data, where applicable) and expiration date.

Disc diameter: 6 mm
Carrier type: Filter paper
Spore population/disc: 106 (1,000,000)
Packaging: Bulk

Excelsior Scientific offers Self-Contained Biological Indicators (SCBI), Mini Self-Contained Biological Indicators (MSCBI) & Rapid Readout Self-Contained Biological Indicators (RSCBI) for monitoring steam (spore population = 105 and 106), Ethylene Oxide and Hydrogen Peroxide (population 106) sterilization processes.

Each SCBI unit consists of a plastic container with a cap, a crushable glass ampule with recovery media, and a disc inoculated with spores.

Excelsior SCBIs are offered in boxes of 50 units, and MSCBIs in boxes of 100 units. They are certified for species, population, purity, resistance (dvalue, zvalue, where applicable) and expiration date.

Sterilisation Process: Steam
Organism: Geobacillus stearothermophilus
Population: 105
Min. Incubation Period: 30 seconds

Excelsior Scientific offers Self-Contained Biological Indicators (SCBI), Mini Self-Contained Biological Indicators (MSCBI) & Rapid Readout Self-Contained Biological Indicators (RSCBI) for monitoring steam (spore population = 105 and 106),Ethylene Oxide and Hydrogen Peroxide (population 106) sterilization processes.

Each SCBI unit consists of a plastic container with a cap, a crushable glass ampule with recovery media, and a disc inoculated with spores.

Excelsior SCBIs are offered in boxes of 50 units, and MSCBIs in boxes of 100 units. They are certified for species, population, purity, resistance (dvalue, zvalue, where applicable) and expiration date.

Sterilisation Process: Steam
Organism: Geobacillus stearothermophilus
Population: 106
Min. Incubation Period: 30 seconds

Mini Self-Contained Biological Indicator
Sterilisation Process: Steam
Organism: Geobacillus stearothermophilus
Population: 105
Min. Incubation Period: 10 hours

Excelsior Scientific offers Self-Contained Biological Indicators (SCBI), Mini Self-Contained Biological Indicators (MSCBI) & Rapid Readout Self-Contained Biological Indicators (RSCBI) for monitoring steam (spore population = 105 and 106),Ethylene Oxide and Hydrogen Peroxide (population 106) sterilization processes.

Each SCBI unit consists of a plastic container with a cap, a crushable glass ampule with recovery media, and a disc inoculated with spores.

Excelsior SCBIs are offered in boxes of 50 units, and MSCBIs in boxes of 100 units. They are certified for species, population, purity, resistance (dvalue, zvalue, where applicable) and expiration date.

Mini Self-Contained Biological Indicator

Sterilisation Process: Steam
Organism: Geobacillus stearothermophilus
Population: 106
Min. Incubation Period: 10 hours

Excelsior Scientific offers Self-Contained Biological Indicators (SCBI), Mini Self-Contained Biological Indicators (MSCBI) & Rapid Readout Self-Contained Biological Indicators (RSCBI) for monitoring steam (spore population = 105 and 106),Ethylene Oxide and Hydrogen Peroxide (population 106) sterilization processes.

Each SCBI unit consists of a plastic container with a cap, a crushable glass ampule with recovery media, and a disc inoculated with spores.

Excelsior SCBIs are offered in boxes of 50 units, and MSCBIs in boxes of 100 units. They are certified for species, population, purity, resistance (dvalue, zvalue, where applicable) and expiration date.

Mini-Spore Ampoules
Geobacillus stearothermophilus 24 month shelf-life
Population: 106
Description: 0.7mL
Packaging: 50/box + 5 negative controls

Excelsior offers spore ampoules for use in monitoring the efficacy of steam sterilisation processes. Spore ampoules are a self contained biological indicator ideal for use in validation of liquid sterilisation cycles. Ampoules may be placed in larger containers such as vials but are also suitable for monitoring non-liquid loads. No activation is required after exposure of the spore ampoule to a sterilisation process. Remove the ampoule post exposure from the steriliser and incubate.

Spore ampoules are manufactured using hermetically sealed Type I borosilicate glass containing a modified Soybean Casein Digest Broth with pH indicator & the specified population of Geobacillus stearothermophilus. Growth is evident by either turbidity and/or a shift in colour.

Spore ampoules are labelled with 24 months of shelf life. Store ampoules under refrigerated conditions (5 ± 3˚C). Spore ampoules are compliant with ANSI/AAMI/ISO/EN and USP where applicable.

Spore ampoules are available in several configurations.

Geobacillus stearothermophilus 24 month shelf-life
Population: 105
Product Description: 1 mL 
Packaging: 50/box

Spore Ampoules are Self-Contained Biological Indicators for monitoring Steam processes consisting of a hermetically sealed glass ampoule which contains modified Tryptic Soy Broth (TSB) with a pH indicator and Geobacillus stearothermophilus (Cell Line 7953) with a population level of 10⁴, 10⁵ or 10⁶. The modified TSB will transition from the initial purple colour to yellow and/or demonstrate turbidity in the presence of bacterial growth.

Spore ampoule
Population: 106
Product description: 1 mL
Packaging: 50/box

Geobacillus stearothermophilus 24 month shelf-life

Excelsior offers spore ampoules for use in monitoring the efficacy of steam sterilization processes. Spore ampoules are ideal for use in validation of liquid sterilisation cycles. No activation is required; simply expose spore ampoule to sterilization process and incubate. Spore ampoules are available in several configurations.

All spore ampoules are hermetically sealed type I borosilicate glass containing a modified Soybean Casein Digest Broth with bromocresol purple pH indicator and the specified population of Geobacillus stearothermophilus spores. Growth is evident by either turbidity and/or a colour shift from a purple to or toward a yellow colour.

Negative Control

Product description: 1 mL control
Packaging: 10/box

24 month shelf-life

Excelsior offers spore ampoules for use in monitoring the efficacy of steam sterilization processes. Spore ampoules are ideal for use in validation of liquid sterilisation cycles. No activation is required; simply expose spore ampoule to sterilization process and incubate. Spore ampoules are available in several configurations.

All spore ampoules are hermetically sealed type I borosilicate glass containing a modified Soybean Casein Digest Broth with bromocresol purple pH indicator and the specified population of Geobacillus stearothermophilus spores. Growth is evident by either turbidity and/or a colour shift from a purple to or toward a yellow colour.

Excelsior Spore Suspensions are pure suspensions of viable spores from specific organisms of known resistance to various sterilization processes.

Spore Population Per 0.1 mL: 10⁶ (1,000,000)

Bacillus subtilis Cell Line 6633 24 month shelf-life

They are suspended in water for injection and provided in pharmaceutical vials containing 10 mL of suspension. Spore suspensions are available in populations of 10² to 10⁸ spores per 0.1mL for each organism.

Excelsior Spore Suspensions are pure suspensions of viable spores from specific organisms of known resistance to various sterilization processes.

Spore Population Per 0.1 mL: 10⁷ (10,000,000)

Bacillus subtilis Cell Line 6633 24 month shelf-life

They are suspended in water for injection and provided in pharmaceutical vials containing 10 mL of suspension. Spore suspensions are available in populations of 102 to 108 spores per 0.1mL for each organism.

Excelsior Spore Suspensions are pure suspensions of viable spores from specific organisms of known resistance to various sterilization processes.

Spore Population Per 0.1 mL: 10⁸(100,000,000)

Bacillus subtilis Cell Line 6633 24 month shelf-life

They are suspended in water for injection and provided in pharmaceutical vials containing 10 mL of suspension. Spore suspensions are available in populations of 102 to 108 spores per 0.1mL for each organism.

Self-Contained Biological Indicators

Sterilisation Process: Steam
Organism: Geobacillus stearothermophilus
Population: 105
Min. Incubation Period: 10 hours

Excelsior Scientific offers Self-Contained Biological Indicators (SCBI), Mini Self-Contained Biological Indicators (MSCBI) & Rapid Readout Self-Contained Biological Indicators (RSCBI) for monitoring steam (spore population = 105 and 106),Ethylene Oxide and Hydrogen Peroxide (population 106) sterilization processes.

Each SCBI unit consists of a plastic container with a cap, a crushable glass ampule with recovery media, and a disc inoculated with spores.

Excelsior SCBIs are offered in boxes of 50 units, and MSCBIs in boxes of 100 units. They are certified for species, population, purity, resistance (dvalue, zvalue, where applicable) and expiration date.

Self-Contained Biological Indicators

Sterilisation Process: Steam
Organism: Geobacillus stearothermophilus
Population: 10⁶
Min. Incubation Period: 10 hours

Excelsior Scientific offers Self-Contained Biological Indicators (SCBI), Mini Self-Contained Biological Indicators (MSCBI) & Rapid Readout Self-Contained Biological Indicators (RSCBI) for monitoring steam (spore population = 105 and 106),Ethylene Oxide and Hydrogen Peroxide (population 106) sterilization processes.

Each SCBI unit consists of a plastic container with a cap, a crushable glass ampule with recovery media, and a disc inoculated with spores.

Excelsior SCBIs are offered in boxes of 50 units, and MSCBIs in boxes of 100 units. They are certified for species, population, purity, resistance (dvalue, zvalue, where applicable) and expiration date.

Spore Strips (Steam)

Spore Population per Strip: 10⁵ (100,000)

Geobacillus stearothermophilus 30 month shelf-life

Spore strips consist of inoculated filter paper, 6mm x 30mm, packaged in glassine pouches. Spore strips are available in populations from 102 to 108 for each organism.

Excelsior strips are manufactured in shelf packs of 100 with a 24 month shelf-life based on the organism and date of manufacturer when stored under room temperature conditions. Spore strips are certified for species, population, purity, resistance, (dvalue, zvalue, survival/kill, where applicable) and expiration date.

Mini Spore Strips – Steam

Packaging: Bulk                          

Spore Population/Strip: 10⁶ (1,000,000)

Geobacillus stearothermophilus 30 month shelf-life.

Excelsior Mini Spore Strips are manufactured with an 18, 24 or 30 month shelf-life when stored under room temperature conditions. Mini spore strips are certified for species, population, purity, resistance, (dvalue, zvalue, survival/kill data, where applicable) and expiration date. Spore strips are available packaged in glassine envelopes or in bulk.

Mini Spore Strips – Steam

Packaging: Glassine envelopes                    

Spore Population/Strip: 10⁶ (1,000,000)

Geobacillus stearothermophilus 30 month shelf-life.

Excelsior Mini Spore Strips are manufactured with an 18, 24 or 30 month shelf-life when stored under room temperature conditions. Mini spore strips are certified for species, population, purity, resistance, (dvalue, zvalue, survival/kill data, where applicable) and expiration date. Spore strips are available packaged in glassine envelopes or in bulk.

Spore Strips - Steam

Spore population per strip: 10⁶ (1,000,000)

Geobacillus stearothermophilus 30 month shelf-life.

Spore strips consist of inoculated filter paper, 6mm x 30mm, packaged in glassine pouches. Spore strips are available in populations from 10² to 10⁸ for each organism.

Excelsior strips are manufactured in shelf packs of 100 with a 24 month shelf-life based on the organism and date of manufacturer when stored under room temperature conditions. Spore strips are certified for species, population, purity, resistance, (dvalue, zvalue, survival/kill, where applicable) and expiration date.

Spore Suspension - Steam

Geobacillus stearothermophilus Cell Line 7953, 36 month shelf-life

Spore Population Per 0.1 mL: 10⁶ (1,000,000)

Excelsior Spore Suspensions are pure suspensions of viable spores with known resistance characteristics and population levels. The convenience of the Spore Suspensions allows for direct inoculation of products, typically to verify sterility of devices where a traditional BI strip cannot be used. They can also be used for a variety of other microbiological tests including cleaning effectiveness studies, Bioburden percent recovery and Bacteriostasis/Fungistasis (BF) testing.

Excelsior Spore Suspensions are packaged in 10 mL volumes and are sold in pharmaceutical grade glass vials with screw cap and septum which allows for withdrawal of the suspension using either a pipette or a needle and syringe assembly. The Spores are suspended in Water for Injection (WFI) in a variety of population levels standardised per 0.1mL. We are also able to provide spores in WFI with up to 40% ethanol mixture upon request.

Spore Suspension - Steam

Geobacillus stearothermophilus Cell Line 7953, 36 month shelf-life

Spore Population Per 0.1 mL: 10⁷ (10,000,000)

Excelsior Spore Suspensions are pure suspensions of viable spores with known resistance characteristics and population levels. The convenience of the Spore Suspensions allows for direct inoculation of products, typically to verify sterility of devices where a traditional BI strip cannot be used. They can also be used for a variety of other microbiological tests including cleaning effectiveness studies, Bioburden percent recovery and Bacteriostasis/Fungistasis (BF) testing.

Excelsior Spore Suspensions are packaged in 10 mL volumes and are sold in pharmaceutical grade glass vials with screw cap and septum which allows for withdrawal of the suspension using either a pipette or a needle and syringe assembly. The Spores are suspended in Water for Injection (WFI) in a variety of population levels standardised per 0.1mL. We are also able to provide spores in WFI with up to 40% ethanol mixture upon request.

Spore Suspension - Steam

Geobacillus stearothermophilus Cell Line 7953, 36 month shelf-life

Spore Population Per 0.1 mL: 108 (100,000,000)

Excelsior Spore Suspensions are pure suspensions of viable spores with known resistance characteristics and population levels. The convenience of the Spore Suspensions allows for direct inoculation of products, typically to verify sterility of devices where a traditional BI strip cannot be used. They can also be used for a variety of other microbiological tests including cleaning effectiveness studies, Bioburden percent recovery and Bacteriostasis/Fungistasis (BF) testing.

Excelsior Spore Suspensions are packaged in 10 mL volumes and are sold in pharmaceutical grade glass vials with screw cap and septum which allows for withdrawal of the suspension using either a pipette or a needle and syringe assembly. The Spores are suspended in Water for Injection (WFI) in a variety of population levels standardised per 0.1mL. We are also able to provide spores in WFI with up to 40% ethanol mixture upon request.

Spore Suspensions - Steam

Spore Population Per 0.1 mL: 10⁶ (1,000,000)

Bacillus Subtilis Cell Line 35021 24 month shelf-life

Excelsior Spore Suspensions are pure suspensions of viable spores with known resistance characteristics and population levels. The convenience of the Spore Suspensions allows for direct inoculation of products, typically to verify sterility of devices where a traditional BI strip cannot be used. They can also be used for a variety of other microbiological tests including cleaning effectiveness studies, Bioburden percent recovery and Bacteriostasis/Fungistasis (BF) testing.

Excelsior Spore Suspensions are packaged in 10 mL volumes and are sold in pharmaceutical grade glass vials with screw cap and septum which allows for withdrawal of the suspension using either a pipette or a needle and syringe assembly. The Spores are suspended in Water for Injection (WFI) in a variety of population levels standardised per 0.1mL. We are also able to provide spores in WFI with up to 40% ethanol mixture upon request.

Spore Suspensions with population levels below 10⁶ are also available upon request; please contact Miclev for pricing, lead time and availability. Excelsior Spore Suspensions are ANSI/AAMI/ISO EN 11138-1 and USP (where applicable) compliant. The Suspensions are labelled with a shelf life based on the organism.

Spore Suspensions require storage under refrigerated conditions (2˚ - 8˚C). Each vial of Spore Suspension is accompanied by a Certificate of Analysis detailing the source, assayed population, resistance characteristics on paper carrier and expiration date.