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LYFO®DISK KWIK STICK™ C-D

Ready-to-use reference stock cultures will alleviate labor-intensive processes of preparing, storing, and maintaining in-house cultures. Each lyophilized microorganism is no more than four passages from the reference culture and is authentic and traceable. Choose from more than 750 microorganism strains derived from well-known culture collections from around the world including ATCC®, NCIMB, NCTC and more. Individual lyophilized microorganisms are available in LYFO DISK® and KWIK-STIK™ configurations. A Certificate of Quality comes standard with each shipment. Also, a printable Certificate of Analysis is available online at www.microbiologics.com 24 hours a day and 7 days a week.
 
*Note: Add the packaging option to the Catalog Number when placing an order: Cat # + L (for LYFO DISK® 6 Pack); Cat # + K (for KWIK-STIK™ 6 Pack); or Cat # + P (for KWIK-STIK™ 2 Pack). Examples are 5041L, 5041K or 5041P. For more information click on the information button for each individual strain.
 
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Campylobacter coli ATCC® 33559™
01023K


Biosafety Level: 2


6 self-contained units of a single organism

Biosafety Level 2
Product FormatKWIK-STIK™ 6 Pack
Test MethodFood and Food Ingredients: Pathogen Testing, Microbial Identification
WDCM Number00072
Catalog number01023
Standards and GuidelinesFDA Bacteriological Analytical Manual (BAM)
TaxonomyBacteria
Industry TypeFood Safety
MediaChocolate Agar
Temperature35°C
AtmosphereMicroaerophilic
Growth Time48 to 72 hours
Additional TipsNote: Do not open the inoculated agar medium petri plate for the first 48 hours.

Campylobacter coli ATCC® 33559™
01023L


Biosafety Level: 2


6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level 2
Product FormatLYFO DISK™
Test MethodFood and Food Ingredients: Pathogen Testing, Microbial Identification
WDCM Number00072
Catalog number01023
Standards and GuidelinesFDA Bacteriological Analytical Manual (BAM)
TaxonomyBacteria
Industry TypeFood Safety
MediaChocolate Agar
Temperature35°C
AtmosphereMicroaerophilic
Growth Time48 to 72 hours
Additional TipsNote: Do not open the inoculated agar medium petri plate for the first 48 hours.

Campylobacter coli ATCC® 33559™
01023P


Biosafety Level: 2


2 self-contained units of a single organism

Biosafety Level 2
Product FormatKWIK-STIK™ 2 Pack
Test MethodFood and Food Ingredients: Pathogen Testing, Microbial Identification
WDCM Number00072
Catalog number01023
Standards and GuidelinesFDA Bacteriological Analytical Manual (BAM)
TaxonomyBacteria
Industry TypeFood Safety
MediaChocolate Agar
Temperature35°C
AtmosphereMicroaerophilic
Growth Time48 to 72 hours
Additional TipsNote: Do not open the inoculated agar medium petri plate for the first 48 hours.

Desulfotomaculum nigrificans ATCC® 7946™*
01026K


Biosafety Level: 1


6 self-contained units of a single organism

Biosafety Level 1
Product FormatKWIK-STIK™ 6 Pack
Test MethodMicrobial Identification
Catalog number01026
TaxonomyBacteria
MediaPhase 1: ISF (modified Infant Soy Formula) Broth, Phase 2: Tranfer to Sulfite Agar
TemperaturePhase 1: 55°C, Phase 2: 55°C
AtmospherePhase 1: Anaerobic, Phase 2: Anaerobic
Growth TimePhase 1: 48 hours, Phase 2: 48 hours to 7 days
Additional TipsPrepare and Use ISF (modified Infant Soy Formula) Broth using the following steps:
1. Fill tubes with 10 ml Infant Soy Formula. Infant Soy Formula may be purchased at a grocery store.
2. Place a four-penny nail in each tube. A four-penny nail is approximately 1.5 inches, or 38 mm, in length. It should contain steel or iron.
3. Sterilize the broth.
4. Inoculate ISF Broth with one LYFO DISK® or KWIK-STIK™.
5. Grow at 55°C in anaerobic conditions for 48 hours. The broth will turn grey, indicating growth.
6. Make two dilutions, 1:10 and 1:100.
7. Sub with a swab to Sulfite Agar. Plate the undiluted sample and the 1:10 and 1:100 dilutions. It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see. Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite.
8. Incubate the agar in anaerobic environment at 55°C for 48 hours to 7 days.

Desulfotomaculum nigrificans ATCC® 7946™*
01026L


Biosafety Level: 1


6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level 1
Product FormatLYFO DISK™
Test MethodMicrobial Identification
Catalog number01026
TaxonomyBacteria
MediaPhase 1: ISF (modified Infant Soy Formula) Broth, Phase 2: Tranfer to Sulfite Agar
TemperaturePhase 1: 55°C, Phase 2: 55°C
AtmospherePhase 1: Anaerobic, Phase 2: Anaerobic
Growth TimePhase 1: 48 hours, Phase 2: 48 hours to 7 days
Additional TipsPrepare and Use ISF (modified Infant Soy Formula) Broth using the following steps:
1. Fill tubes with 10 ml Infant Soy Formula. Infant Soy Formula may be purchased at a grocery store.
2. Place a four-penny nail in each tube. A four-penny nail is approximately 1.5 inches, or 38 mm, in length. It should contain steel or iron.
3. Sterilize the broth.
4. Inoculate ISF Broth with one LYFO DISK® or KWIK-STIK™.
5. Grow at 55°C in anaerobic conditions for 48 hours. The broth will turn grey, indicating growth.
6. Make two dilutions, 1:10 and 1:100.
7. Sub with a swab to Sulfite Agar. Plate the undiluted sample and the 1:10 and 1:100 dilutions. It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see. Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite.
8. Incubate the agar in anaerobic environment at 55°C for 48 hours to 7 days.

Desulfotomaculum nigrificans ATCC® 7946™*
01026P


Biosafety Level: 1


2 self-contained units of a single organism

Biosafety Level 1
Product FormatKWIK-STIK™ 2 Pack
Test MethodMicrobial Identification
Catalog number01026
TaxonomyBacteria
MediaPhase 1: ISF (modified Infant Soy Formula) Broth, Phase 2: Tranfer to Sulfite Agar
TemperaturePhase 1: 55°C, Phase 2: 55°C
AtmospherePhase 1: Anaerobic, Phase 2: Anaerobic
Growth TimePhase 1: 48 hours, Phase 2: 48 hours to 7 days
Additional TipsPrepare and Use ISF (modified Infant Soy Formula) Broth using the following steps:
1. Fill tubes with 10 ml Infant Soy Formula. Infant Soy Formula may be purchased at a grocery store.
2. Place a four-penny nail in each tube. A four-penny nail is approximately 1.5 inches, or 38 mm, in length. It should contain steel or iron.
3. Sterilize the broth.
4. Inoculate ISF Broth with one LYFO DISK® or KWIK-STIK™.
5. Grow at 55°C in anaerobic conditions for 48 hours. The broth will turn grey, indicating growth.
6. Make two dilutions, 1:10 and 1:100.
7. Sub with a swab to Sulfite Agar. Plate the undiluted sample and the 1:10 and 1:100 dilutions. It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see. Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite.
8. Incubate the agar in anaerobic environment at 55°C for 48 hours to 7 days.

Candida tropicalis ATCC® 1369™
01036K


Biosafety Level: 1


6 self-contained units of a single organism

Biosafety Level 1
Product FormatKWIK-STIK™ 6 Pack
Strain Characteristicsyeast cells
Test MethodMicrobial Identification
Catalog number01036
TaxonomyFungi
MediaSabouraud Dextrose Emmons Agar
Temperature25°C
AtmosphereAerobic
Growth Time2 to 7 days
Additional TipsNonselective Sheep Blood Agar is an appropriate alternative.
Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

Candida tropicalis ATCC® 1369™*
01036L


Biosafety Level: 1


6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level 1
Product FormatLYFO DISK™
Strain Characteristicsyeast cells
Test MethodMicrobial Identification
Catalog number01036
TaxonomyFungi
MediaSabouraud Dextrose Emmons Agar
Temperature25°C
AtmosphereAerobic
Growth Time2 to 7 days
Additional TipsNonselective Sheep Blood Agar is an appropriate alternative.
Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

Candida tropicalis ATCC® 1369™
01036P


Biosafety Level: 1


2 self-contained units of a single organism

Biosafety Level 1
Product FormatKWIK-STIK™ 2 Pack
Strain Characteristicsyeast cells
Test MethodMicrobial Identification
Catalog number01036
TaxonomyFungi
MediaSabouraud Dextrose Emmons Agar
Temperature25°C
AtmosphereAerobic
Growth Time2 to 7 days
Additional TipsNonselective Sheep Blood Agar is an appropriate alternative.
Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

Corynebacterium urealyticum ATCC® 43044™*
01039K


Biosafety Level: 2


6 self-contained units of a single organism

Biosafety Level 2
Product FormatKWIK-STIK™ 6 Pack
Test MethodMicrobial Identification
Catalog number01039
TaxonomyBacteria
MediaNonselective Sheep Blood Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 72 hours

Corynebacterium urealyticum ATCC® 43044™*
01039L


Biosafety Level: 2


6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level 2
Product FormatLYFO DISK™
Test MethodMicrobial Identification
Catalog number01039
TaxonomyBacteria
MediaNonselective Sheep Blood Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 72 hours

Corynebacterium urealyticum ATCC® 43044™*
01039P


Biosafety Level: 2

2 self-contained units of a single organism

Biosafety Level 2
Product FormatKWIK-STIK™ 2 Pack
Test MethodMicrobial Identification
Catalog number01039
TaxonomyBacteria
MediaNonselective Sheep Blood Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 72 hours

Corynebacterium renale ATCC® BAA-1785™*
01040K


Biosafety Level: 2


6 self-contained units of a single organism

Biosafety Level 2
Product FormatKWIK-STIK™ 6 Pack
Test MethodMicrobial Identification
Catalog number01040
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Corynebacterium renale ATCC® BAA-1785™*
01040L


Biosafety Level: 2


6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level 2
Product FormatLYFO DISK™
Test MethodMicrobial Identification
Catalog number01040
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Corynebacterium renale ATCC® BAA-1785™*
01040P


Biosafety Level: 2

2 self-contained units of a single organism

Biosafety Level 2
Product FormatKWIK-STIK™ 2 Pack
Test MethodMicrobial Identification
Catalog number01040
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Curtobacterium pusillum ATCC® 19096™*
01044K


Biosafety Level: 1


6 self-contained units of a single organism

Biosafety Level 1
Product FormatKWIK-STIK™ 6 Pack
Test MethodMicrobial Identification
Catalog number01044
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Curtobacterium pusillum ATCC® 19096™*
01044L


Biosafety Level: 1


6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level 1
Product FormatLYFO DISK™
Test MethodMicrobial Identification
Catalog number01044
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Curtobacterium pusillum ATCC® 19096™*
01044P


Biosafety Level: 1


2 self-contained units of a single organism

Biosafety Level 1
Product FormatKWIK-STIK™ 2 Pack
Test MethodMicrobial Identification
Catalog number01044
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Cellulosimicrobium cellulans ATCC® BAA-1816™*
01046K


Biosafety Level: 1


6 self-contained units of a single organism

Biosafety Level 1
Product FormatKWIK-STIK™ 6 Pack
Test MethodMicrobial Identification
Catalog number01046
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Cellulosimicrobium cellulans ATCC® BAA-1816™*
01046L


Biosafety Level: 1


6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level 1
Product FormatLYFO DISK™
Test MethodMicrobial Identification
Catalog number01046
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Cellulosimicrobium cellulans ATCC® BAA-1816™*
01046P


Biosafety Level: 1


2 self-contained units of a single organism

Biosafety Level 1
Product FormatKWIK-STIK™ 2 Pack
Test MethodMicrobial Identification
Catalog number01046
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Cellulosimicrobium cellulans ATCC® BAA-1817™*
01047K


Biosafety Level: 1


6 self-contained units of a single organism

Biosafety Level 1
Product FormatKWIK-STIK™ 6 Pack
Test MethodMicrobial Identification
Catalog number01047
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Cellulosimicrobium cellulans ATCC® BAA-1817™*
01047L


Biosafety Level: 1


6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level 1
Product FormatLYFO DISK™
Test MethodMicrobial Identification
Catalog number01047
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Cellulosimicrobium cellulans ATCC® BAA-1817™*
01047P


Biosafety Level: 1


2 self-contained units of a single organism

Biosafety Level 1
Product FormatKWIK-STIK™ 2 Pack
Test MethodMicrobial Identification
Catalog number01047
TaxonomyBacteria
MediaTryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature35°C
AtmosphereAerobic
Growth Time24 to 48 hours

Clostridioides difficile derived from ATCC® BAA-1870™
01048K

6 self-contained units of a single organism

Biosafety Level 2
Product FormatKWIK-STIK™ 6 Pack
Strain CharacteristicsGenotype:
• tcdA positive, tcdB positive
• cdtB positive
Binary Toxin positive
Toxinotype IIIb
Ribotype 027
PFGE Type NAP1
REA type BI 8

Formerly Clostridium difficile
Test MethodMicrobial Identification
Catalog number01048
TaxonomyBacteria
Industry TypeClinical
MediaAnaerobic Blood Agar
Temperature35°C
AtmosphereAnaerobic
Growth Time48 to 72 hours
Additional TipsSome obligate anaerobes may require 5 to 7 days to demonstrate sufficient growth.

Page 1 of 13

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